农药沉积利用率喷雾指示剂诱惑红的酶标仪测定方法

诱惑红是一种水溶性染料,近年来被用作农药沉积利用率测定的指示剂。诱惑红的检测方法为高效液相色谱法和分光光度法。高效液相色谱法需要专门的分析仪器,且价格昂贵,可见分光光度计法操作繁琐,试剂用量大,耗时长,均不适宜田间快速高效测定。笔者发展了诱惑红的酶标仪测定方法,简单高效,可批量检测多个样品,且准确度和精密度高,线性关系良好,514 nm检测波长下不受其他农药影响,并应用于玉米无人飞机喷雾场景下农药雾滴在玉米冠层的沉积利用率测定。     

The Age-Sensitive Efficacy of Calorie Restriction on Mitochondrial Biogenesis and mtDNA Damage in Rat Liver

Calorie restriction (CR) is the most efficacious treatment to delay the onset of age-related changes such as mitochondrial dysfunction. However, the sensitivity of mitochondrial markers to CR and the age-related boundaries of CR efficacy are not fully elucidated. We used liver samples from ad libitum-fed (AL) rats divided in: 18-month-old (AL-18), 28-month-old (AL-28), and 32-month-old (AL-32) groups, and from CR-treated (CR) 28-month-old (CR-28) and 32-month-old (CR-32) counterparts to assay the effect of CR on several mitochondrial markers. The age-related decreases in citrate synthase activity, in TFAM, MFN2, and DRP1 protein amounts and in the mtDNA content in the AL-28 group were prevented in CR-28 counterparts. Accordingly, CR reduced oxidative mtDNA damage assessed through the incidence of oxidized purines at specific mtDNA regions in CR-28 animals. These findings support the anti-aging effect of CR up to 28 months. Conversely, the protein amounts of LonP1, Cyt c, OGG1, and APE1 and the 4.8 Kb mtDNA deletion content were not affected in CR-28 rats. The absence of significant differences between the AL-32 values and the CR-32 counterparts suggests an age-related boundary of CR efficacy at this age. However, this only partially curtails the CR benefits in counteracting the generalized aging decline and the related mitochondrial involvement.     

Investigation on thermal conductivity and mechanical properties of Si3N4 ceramics via one-step sintering

High thermal conductivity Si₃N₄ ceramics were fabricated using a one-step method consisting of reaction-bonded Si₃N₄ (RBSN) and post-sintering. The influence of Si content on nitridation rate, β/(α+β) phase rate, thermal conductivity and mechanical properties was investigated in this work. It is of special interest to note that the thermal conductivity showed a tendency to increase first and then decrease with increasing Si content. This experimental result shows that the optimal thermal conductivity and fracture toughness were obtained to be 66 W (m K)^-1 and 12.0 MPa m^1/2, respectively. As a comparison, the nitridation rate and β/(α+β) phase rate in a static pressure nitriding system, i.e., 97% (MS10), 97% (MS15), 97% (MS20) and 8.3% (MS10), 8.3% (MS15), 8.9% (MS20), respectively, have obvious advantages over those in a flowing nitriding system, i.e., 91% (MS10), 91% (MS15), 93% (MS20) and 3.1% (MS10), 3.3% (MS15), 3.3% (MS20), respectively. Moreover, high lattice integrity of the β-Si₃N₄ phase was observed, which can effectively confine O atoms into the β-Si₃N₄ lattice using MgO as a sintering additive. This result indicates that one-step sintering can provide a new route to prepare Si₃N₄ ceramics with a good combination of thermal conductivity and mechanical properties.     

Optimizing the stability and electrical transport properties of CeNbO4+δ-based oxide ceramics by regulating oxygen ion content

CeNbO_4+δ ceramics have attracted extensive research interest because of their unique mixed ion-electron transport characteristics and interesting structure-functional characteristics caused by the difference in oxygen ion content. Although the change of oxygen ion content brings rich redox properties, it also causes serious crystal transformation and abnormal electrical transport properties. In order to obtain stable structure and excellent electrical transport properties, the directional regulation of the oxygen ion content has been realized through introducing Al₂O₃ and high temperature aging. After 600 h of aging at 1073 K, the prepared composite ceramics not only obtain a stable structure without crystal transformation, but also show good negative temperature coefficient (NTC) thermistor characteristics in the temperature range of 473 K–1273 K, in which the linear fitting maximum Pearson's r of the relationship between lnρ and 1000/T can reach 99.97%. The proposed method provides a new thought for the design and application of high-temperature electronic ceramics.     

Corrosion behaviour of an industrial shot-peened and coated automotive spring steel AISI 9254

A high-strength low-alloy steel, AISI 9254 (54SiCr6), is widely used for suspension spring production in the automotive industry. In this work, industrially manufactured zinc phosphate coated helical springs are subjected to detailed microstructural and surface analysis for better understanding of corrosion evolution. The material’s free corrosion potential and anodic/cathodic behaviour were investigated in NaCl solutions and corrosion propagation mechanisms were studied using potentiostatic polarisation on cross-sectional and external surfaces. The bulk material is fully martensite with uniformly distributed MnS inclusions, while the spring surface has a 2–3?μm mechanically deformed region introduced by shot-peening and a thin zinc phosphate coating. The corrosion open circuit potential of bulk material and shot-peened spring surface was about –0.7V_SCE without significant difference, while phosphated surface is more noble (more positive potential). MnS inclusions, stimulating the anodic attack in the steel, influence corrosion propagation and pit morphology to a large extent that can have an impact on the spring performance.     

A comparative study on pyrolysis reactivity and gas release characteristics of biomass and coal using TG-MS analysis

Pyrolysis reactivity and gas release characteristics of biomass and coals were comparatively investigated using TG-MS. In addition, chemical compositions and structures of tested samples were determined to correlate pyrolysis gas release characteristic. It was found that pyrolysis reactivity and gaseous product release were hardly varied with pyrolysis heating rate. Furthermore, pyrolysis rates of two biomass samples were greater than those of coals, which were mainly attributed to the chemical compositions and the gaseous product evolution process. The result also showed that gaseous product release during pyrolysis process was related to the presence of different functional groups in samples.     

低阶煤颗粒-气/油泡间的诱导时间研究

为表征低阶煤颗粒-气/油泡间矿化过程的差异,通过Sutherland理论下固体颗粒进入泡沫产品的总概率(E)和浮选速率常数(k)之间关系,并结合低阶煤颗粒-气/油泡的浮选速率试验,求得了低阶煤颗粒-气/油泡间的诱导时间。浮选实验研究表明,在相同的捕收剂消耗量下低阶煤-油泡浮选产率均高于低阶煤-气泡浮选产率。诱导时间测试表明,低阶煤颗粒-油泡间的诱导时间(35 ms)要明显低于低阶煤颗粒-气泡间的诱导时间(93 ms)。上述实验结果表明,油泡表面的疏水性要强于传统浮选气泡表面的疏水性。然而,进一步利用Sutherland理论中固体颗粒进入泡沫产品的总概率和浮选速率常数之间的数学关系,并结合低阶煤颗粒-气/油泡的浮选速率试验求得的低阶煤颗粒-气/油泡间的诱导时间分别为9.67和8.46 ms,其与诱导时间测试仪分别测量的诱导时间差异很大。这主要是由于在实际浮选过程中气/油泡的上升速度分别为23.26和22.68 cm/s,其远高于2015EZ型诱导时间仪测试过程中气/油泡碰撞速度(2.0 cm/s)。因此,诱导时间理论计算表明气泡-颗粒间的碰撞速度对颗粒-气泡间的诱导时间影响很大。上述研究结果表明油泡浮选效果优于传统浮选的内在原因在于低阶煤颗粒-油泡间的诱导时间小于低阶煤颗粒-气泡间的诱导时间。     

Viscoelastic Behavior of Synthesized Liquid Soaps and Surface Activity Properties of Surfactants

In the present work, a rheological study of liquid soaps prepared from different mixture of surfactants as a function of surfactant type and concentration was performed. The curves of shear stress vs. shear rate and viscosity vs. shear rate were recorded at constant temperature, 294 ± 0.1 K. The surface activity properties were also studied. The results of the study showed that values of surface tension, γ, were in the range 31–40 mN m⁻¹ and the critical micelle concentration (CMC), was of the order 10⁻⁴ mol L⁻¹. The calculated maximum surface excess, Γ_max, varied from 2.40 to 3.66 μmol m⁻², while minimum area per molecule, A_min, varied from 41.1 (for amphoterics) to 81.4 Ų (for nonionic surfactants). The standard free energy of micellization, −29.8 and −29.3 kJ mol⁻¹ for anionic and amphoteric surfactants, respectively, were while values for nonionic surfactants varied between −31.8 and − 30.3 kJ mol⁻¹. The free energy of adsorption, was the lowest for amphoteric surfactants (−37.9 kJ mol⁻¹), followed by anionics (−40.4 kJ mol⁻¹) and nonionics (−43.34 to −46.84 kJ mol⁻¹), indicating that micellization process is spontaneous in the examined medium. The synthetized liquid soaps show pseudoplastic behavior and they achieved pipe flow. The results of this research indicate that flow behavior was affected significantly by the ionic charge of the surfactant and the ionic strength of the formulation, suggesting that the flow behavior could be changed by manipulating the choice of the surfactant and salinity. The pH value of all liquid soaps examined were weakly acidic, in the range of 5.0–6.4.     

等通道挤压纯铝的冷轧织构与组织演变

研究了冷轧形变量对等通道挤压纯铝的形变织构与显微组织的影响。结果表明：当形变量较小时，冷轧使挤压高纯铝的显微组织分布更加均匀。然而，随着形变量的增加，晶粒由等轴向纤维状转变。织构的演变规律为旋转立方织构和{111}<110>向冷轧织构（铜织构、黄铜织构和S织构）转变，同时出现了R织构。由此可见，等通道挤压变形对高纯铝的冷轧组织和织构存在一定的影响。